Executive (B)

Yashraj Biotech is seeking a highly motivated Scientist with 4–5 years of industrial experience in antibody bioconjugation, immunoassay development, and diagnostic reagent development. The candidate will work in the development and optimization of antibody-latex conjugates for immunoturbidimetric assays while collaborating with multidisciplinary R&D and Quality teams to deliver robust diagnostic products.

Key Responsibilities

• Design, execute, and optimize antibody purification, characterization, and bioconjugation workflows.
• Develop EDC/NHS-based covalent conjugation strategies for latex and nanoparticle-based diagnostic reagents.
• Optimize antibody loading, blocking conditions, coupling chemistry, and conjugate stability.
• Develop and optimize immunoturbidimetric assays (ITA)
• Design and execute DOE-based optimization studies for conjugation efficiency and assay performance.
• Evaluate analytical performance including sensitivity, specificity, linearity, precision, dynamic range, and robustness.
• Troubleshoot conjugation, assay performance, and reagent stability issues.
• Maintain accurate experimental documentation, SOPs, technical reports, and development records in compliance with GLP.
• Mentor junior scientists, trainees, and interns in laboratory techniques and experimental planning.
• Participate in technology transfer activities and support manufacturing scale-up of developed reagents.

Cross-Functional Responsibilities

• Collaborate with Polyclonal and Monoclonal Antibody R&D teams for antibody selection and characterization.
• Work closely with Quality Control (QC) during analytical validation and product performance evaluation.
• Coordinate with Quality Assurance (QA) to ensure compliance with documentation, change control, and regulatory requirements.
• Support Manufacturing in process optimization and troubleshooting during scale-up.
• Participate in product development meetings, technical reviews, and root-cause investigations.
• Contribute to continuous process improvement and new technology implementation.

Required Technical Skills Antibody & Protein Sciences

• Strong understanding of monoclonal, polyclonal, artificial polyclonal antibodies.
• Knowledge of antibody structure, affinity, avidity, stability, and antigen-antibody interaction kinetics.
• Experience handling native and recombinant antigens and antibodies.
• Hands-on experience in antibody purification using ammonium sulphate precipitation, dialysis, ultrafiltration, and related techniques.
• Understanding of protein stability and conjugate characterization.

Bioconjugation

• Hands-on industrial experience in EDC/NHS chemistry, Maleimide–Thiol Chemistry, Glutaraldehyde Coupling
• Expertise in antibody immobilization onto latex beads , nanoparticles
• Understanding of surface chemistry, functional group activation, antibody orientation, and conjugation efficiency
• Experience in optimization of conjugation parameters for improved assay performance

Latex/Nanoparticle Diagnostics

• Experience developing latex-based diagnostic reagents.
• Understanding of particle size selection, surface modification, colloidal stability, blocking strategies, and storage stability.
• Knowledge of reagent formulation and long-term stability assessment.

Immunoassays

• Hands-on experience with Immunoturbidimetric Assays (ITA)
• Working knowledge of ELISA and CLIA platforms
• Experience in assay optimization, calibration curve generation, cut-off determination, and analytical validation

Analytical Techniques Experience With

• Immunoturbidometry
• UV-Visible Spectrophotometry
• Downstream processing of polyclonal antibodies
• Dynamic Light Scattering (DLS)
• Zeta potential analyser
• Dialysis and ultrafiltration
• Statistical analysis of assay performance

Laboratory Practices

• Excellent laboratory skills including precision pipetting and sample handling.
• Experience working under GLP/GMP environments.
• Knowledge of laboratory safety, contamination control, and documentation practices.

Desired Competencies

• Strong experimental design and scientific problem-solving skills.
• Excellent troubleshooting and analytical thinking.
• Ability to independently manage multiple R&D projects.
• Strong written and verbal scientific communication.
• Experience mentoring junior team members.
• Good organizational and project management skills.
• Ability to work effectively in cross-functional teams.