Research Technologist 2, Pulmonary

Department: MED-Pulmonary

Salary/Grade: NEX/11

Job Summary Conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s).May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data.Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices

Specific Responsibilities Molecular Biology:

• DNA, RNA, Protein isolation, purification and expressionSouthern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA
• Recombinant virus production and purification

Cellular Biology

• Cell culture, cell isolation
• Functional studies
• Transfection

Histology

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry
• Microscopy

Immunology

• Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification

Animal

• Husbandry
• Breedingand screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony

Miscellaneous Performs other duties as assigned.

Minimum Qualifications

• Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor’s or higher degree in a scientific major or related; OR appropriate combination of education and experience.

Minimum Competencies: (Skills, knowledge, and abilities.)

• Demonstrated laboratory experience;
• Demonstrated ability to follow detailed instructions as required by research protocols;
• Demonstrated computer skills, including spreadsheets and basic computations;
• Attention to detail and accurate record keeping;
• Ability to work independently and in a team environment;
• Manual dexterity.

Target hiring range for this position will be between $19.89 - 27.97 per hour. Offered salary will be determined by the applicant’s education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.

Benefits At Northwestern, we are proud to provide meaningful and competitive benefits. The available benefits package for this position will include health, dental, vision, disability, and life insurance; paid vacation and holidays; paid medical/sick and parental leave; tuition benefits for the employee and dependents; pre-tax and flex spending accounts for commuting and dependent care; generous retirement savings options; and wellness programs. For a comprehensive overview of available benefits, including eligibility details, visit us at https://www.northwestern.edu/hr/benefits/index.html to learn more.

Work-Life And Wellness Northwestern offers comprehensive programs and services to help you and your family navigate life’s challenges and opportunities, and adopt and maintain healthy lifestyles. We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at https://www.northwestern.edu/hr/benefits/work-life/index.html to learn more. Professional Growth & Development Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you’re interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at https://www.northwestern.edu/hr/learning/index.html to learn more . Northwestern University is an Equal Opportunity Employer and does not discriminate on the basis of protected characteristics, including disability and veteran status. View Northwestern’s non-discrimination statement . Job applicants who wish to request an accommodation in the application or hiring process should contact the Office of Civil Rights and Title IX Compliance. View additional information on the accommodations process .