北京蜜莱坞网络科技有限公司
蛋白纯化 助理研究员 / 工程师
12 days ago
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Important information
Contract type:
Permanent
Salary:
CN¥120,000 – CN¥360,000 / year
Location:
Shanghai, China
Work mode:
Onsite
Published on:
11 October 2026
What they need
该职位来源于猎聘 【核心岗位职责】 1. 细胞培养与重组蛋白表达(Cell Culture & Expression Execution): o 负责 HEK293、CHO 等哺乳动物细胞的传代、扩增、高密度瞬时转染及上清收集; o 协助维护昆虫细胞或大肠杆菌表达系统,执行摇瓶或小型生物反应器的日常发酵。 2. 重组蛋白与抗体分离纯化(Protein & Antibody Purification): o 熟练操作 ÄKTA 纯化系统或重力柱/磁珠系统,执行亲和层析(Protein A/G、Ni-NTA、Flag/His tag)及常规分子筛(SEC)分离; o 负责样品的浓缩、超滤置换、除菌过滤、分装与深低温冻存管理。 3. 常规质量检测与数据记录(Routine Analytical QC & Records): o 负责样品的 SDS-PAGE、Western Blot、Nanodrop/BCA 浓度定量等常规质检; o 进行 SEC-HPLC 或内毒素(Endotoxin)检测,准确记录各批次样品的收率、纯度与批号; o 及时撰写并提交电子实验记录,对样品库存信息进行数字化录入与追踪。 4. 实验室仪器与耗材维护(Lab Maintenance): o 负责 ÄKTA 纯化系统的日常维护、管路清洗、色谱柱清洗(CIP)与填料再生再生保养; o 协助管理实验室常用缓冲液配制、试剂耗材盘点与采购申请。
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