Chiara Caiusi

– Program management of one of seven Integrated Biologics R&D Capabilities, managing projects that empower manufacturing decision making through advanced AI and data insights, and digital infrastructure setup – Project management of a selection of Integrated Biologics R&D portfolio projects, from proof of concept to handover to Operations for implementation – Maintenance of project documentation and overseeing governance adherence throughout project initiation, execution and closing – Concurrent Lean Waterfall project management of a selection of R&D and operational projects – Designed and delivered a five month fortnightly course to coach a junior colleague towards passing their PRINCE2 qualification – Tailored, presented and supported implementation of Agile practises in our Bioinformatics team – Pilot tested changes in governance to a more 'light touch' approach to project management, ensuring lessons learned were captured, key metrics tracked and outcomes from this governance change fed back to senior level

– Project and program management of complex tech projects in energy, FMCG and transport – Partnered with C-suite and senior leaders to drive clarity and execution on complex Agile tech projects, actively working with clients and SMEs to focus on tangible business value – Delivering in a high-uncertainty environment: risk management, escalation, assessment and mitigation part of daily tasks – Drafted and reviewed contracts ahead of bidding/work proposals, point of contact for clients – Owned project budgets and commercial forecast, ensuring deliverables met strict milestones – Drove requirements gathering and selection committee of new PM software – Implemented use of project dashboards within the PM team processes to facilitate onboarding of new team member – Successfully delivered two client projects leading to follow on work

Specialties: – Protein Purification – GMP assay performance and evaluation (CE-SDS, GXII Caliper), working knowledge of HPLC/IEF – Thorough knowledge and troubleshooting of specialized equipment (PA800, GXII Touch) – Fully trained in use of specialized software (Empower, 32 Karat, LabChip GXII) – Experienced E-Workbook Suite, IFS, SAP user; Assay report/data analysis reviewer – Fully responsible for laboratory stock check maintenance and budgeting – Buffer laboratory owner and 6S area lead – Health and Safety Focal Point, First Aider – Supplier representative interface and engineer point of contact

Specialties: – Experienced and fully trained in Containment Level 3 (CL3) practices, GMP and GDP – Fully trained in CL3 assays (Replication Competent Lentivector assay) – Experience of validation and verification studies (automation and tailoring RCL assays to individual clients) – Trainer in aseptic technique, tissue and cell culture, responsible for operator scheduling and passaging plans – Stock, consumables and equipment maintenance – Fully trained in qPCR (Product Enhanced Reverse Transcription assay), LacZ staining – On the day and night-time call out team for the Facilities Monitoring System – Safety Auditor and safety representative for the CL3 team (training provided by Key OSTAS) – Fully trained first aider and fire marshal – Quality Assurance and Control plays a large part of day to day activities – Completion of Quality Incidents, Deviations, CAPAs and Change Controls to high standards – Lengthy experience updating or writing Standard Operating Procedures and associated forms, Risk Assessments and Codes of Practice – Involved in FDA, HSE, and client-based audits

Working on the Rapidia-FIELD project on the use of LAMP (Loop Mediated Isothermal Amplification) to identify livestock animals with Foot and Mouth Disease or Swine Vesicular Disease. More specifically, tested and optimized the published versions of the LAMP assay for the two viruses, adapted the assay to be run on the portable Genie II machine for use on the field, and set up a multiplex assay to test for both FMDV and SVDV via LAMP and Lateral Flow Device using fluorescence labelled primers. On the side, compared and analyzed new sequences found in this laboratory for SVDV, performed a sliding window analysis (SWAN) for variability and confirmed that the primers being used for the SVDV LAMP were still the best ones according to the new sequences. Key points: – High containment laboratory rules and SOPs – Multiplexing Foot-and-Mouth Disease and Swine Vesicular Disease – Primer design and alignment – RNA extraction from supernatant via purification kits – RT-PCR and RT-LAMP – Dry down assay for RT-PCR – Gel electrophoresis – Lateral Flow Device – Sensitivity tests – RNA quantitation via NanoDrop – Assay Development and Optimization Data presented at EUFMD Croatia 2014

This job entitles researching internet services websites on the web and classifying the services they offer to their clients. It is very challenging, as all the work is done on a form provided, and the researcher must find all the details for the specific company, searching the site extensively and sometimes searching for more information on the internet

Four weeks of apprenticeship and hands on experience in four different laboratory sectors: cell cultures, GMO testing, bacterial virology, and DNA sequencing. The internship was very educational, as I learned both the theory behind the use of certain techniques, and how to use the techniques themselves, along with the appropriate machines involved in DNA sequencing and PCR reactions